ProductName: | RecombinantHumanLIFProtein |
Description: | LeukemiaInhibitoryFactor(LIF)isalymphoidfactorwhichpromoteslong-termmaintenanceofpluripotentembryonicstemcellsbysuppressingspontaneousdifferentiation.LeukemiaInhibitoryFactor(LIF)hasanumberofotherfunctionsincludingcholinergicneurondifferentiation,boneandfatmetabolism,mitogenesisofcertainfactordependentcelllinesandpromotionofmegakaryocyteproductioninvivo.HumanLIFhasamolecularmassof20kDacontaining181aminoacidresiduesandisactiveonbothhumanandmousecells.MouseLIFisabout1000foldlessactiveonhumancells,thanhumanLIF. |
AvailableSizes: | 10ug(10^6units/ml),100ug(10^7units/ml) |
CatalogNumber: | 12-0002-10,12-0002-100 |
Source: | EscherichiaColi. |
Purity: | Greaterthan98%,asdeterminedbySDS-PAGE |
Endotoxin: | Lessthan0.01ng/μgcytokineasdeterminedbytheLALassay |
Storage: | Thisproductisshippedatambienttemperaturewithcoldpack.Itisstableforupto6monthsfromdateofreceiptwhenstoredat4°C.Freeze-thawshouldbeavoidedasitcanresultinlossofactivity. |
Formulation: | HumanLIFisa0.22μmfilteredsterileliquidinphosphate-bufferedsalinewith0.02%Tween-20and1%BSA. |
SuggestedUsage: | 1mlofhumanLIFwiththespecificactivityof1x106units/mlissufficienttotreat1Lofembryonicstemcellculturemedia. 1mlofhumanLIFwiththespecificactivityof1xunits/mlissufficienttotreat10Lofembryonicstemcellculturemedia. |